02344nas a2200157 4500008004100000245016500041210006900206490000700275520168500282653010401967100001502071700001402086700001902100700002002119856004702139 2014 eng d00aNon-monophyly of Buglossoides (Boraginaceae: Lithospermeae): Phylogenetic and morphological evidence for the expansion of Glandora and reappraisal of Aegonychon0 aNonmonophyly of Buglossoides Boraginaceae Lithospermeae Phylogen0 v633 a

The phylogeny of the small Old World genus Buglossoides and its position in tribe Lithospermeae was investigated
using nrDNA and cpDNA sequences and morphology. Maximum parsimony and Bayesian analyses of ITS-5.8S and trnL-trnF
IGS datasets consistently show that this group is close to Glandora and Lithospermum but not monophyletic. Of the seven spe-
cies usually included, two were retrieved in the genus Glandora, i.e., B. goulandrisiorum from northern Greece and B. gastonii
from the western Pyrenees. Based also on morphology and ecology, the placement of these two rare, rupicolous endemics in
Glandora is here advocated and new combinations are made. The rest of Buglossoides includes two early-diverging clades,
one with annual taxa of section Buglossoides and one with the three perennials of section Margarospermum. Morphological,
palynological and ecological data support the separation of these two groups in distinct genera, Buglossoides s.str. and the
old but largely neglected Aegonychon. Within Buglossoides, two main clades correspond to the B. arvensis and B. incrassata
complexes. These show a largely sympatric distribution from the south Mediterranean to central and northern Europe. Com-
bined with their strong phenotypic polymorphism, this causes difficulties in the distinction between taxa of the two clades,
especially without characteristic cotyledons or fruiting material. Molecular and morphological evidence clearly support the
transfer of the west Mediterranean B. arvensis subsp. permixta to the B. incrassata complex.

 

10aAegonychon; Boraginaceae; Buglossoides; Glandora; ITS; morphology; pollen; phylogeny; trnL-trnF IGS1 aCecchi, L.1 aCoppi, A.1 aHilger, H., H.1 aSelvi, Federico uhttps://boraginales.myspecies.info/node/8800581nas a2200193 4500008004100000245007600041210006700117300001200184490000700196100001500203700002200218700002000240700001600260700001900276700001500295700001400310700001600324856004700340 2005 eng d00aThe {Euro+Med} treatment of Boraginaceae in Willdenowia 34 - a response0 aEuroMed treatment of Boraginaceae in Willdenowia 34 a response a43–480 v351 aHilger, HH1 aGottschling, Marc1 aSelvi, Federico1 aBigazzi, M.1 aLangström, E.1 aZippel, E.1 aDiane, N.1 aWeigend, M. uhttps://boraginales.myspecies.info/node/5800542nas a2200157 4500008004100000245012500041210006900166300001400235490000800249100002000257700001500277700001500292700001600307700001400323856004700337 2004 eng d00aThe phylogenetic relationships of Cynoglottis (Boraginaceae-Boragineae) inferred from {ITS}, {5.8S} and {trnL} sequences0 aphylogenetic relationships of Cynoglottis BoraginaceaeBoragineae a195–2090 v2461 aSelvi, Federico1 aPapini, A.1 aHilger, HH1 aBigazzi, M.1 aNardi, E. uhttps://boraginales.myspecies.info/node/5200582nas a2200157 4500008004100000245015800041210006900199300001400268490000700282653002100289100002000310700001600330700001500346700001600361856004700377 2006 eng d00aMolecular phylogeny, morphology and taxonomic re-circumscription of the generic complex {Nonea/Elizaldia/Pulmonaria/Paraskevia} (Boraginaceae-Boragineae)0 aMolecular phylogeny morphology and taxonomic recircumscription o a907–9180 v5510aBoraginales 20131 aSelvi, Federico1 aBigazzi, M.1 aHilger, HH1 aPapini, Al. uhttps://boraginales.myspecies.info/node/5300526nas a2200145 4500008004100000245013600041210006900177300001400246490000700260100001500267700002000282700001500302700001600317856004700333 2004 eng d00aMolecular systematics of Boraginaceae tribe Boragineae based on {ITS1} and {trnL} sequences, with special reference to Anchusa s.l.0 aMolecular systematics of Boraginaceae tribe Boragineae based on  a201–2120 v941 aHilger, HH1 aSelvi, Federico1 aPapini, A.1 aBigazzi, M. uhttps://boraginales.myspecies.info/node/5500822nas a2200157 4500008004100000245014500041210006900186260001200255300001000267490000600277520027200283100001500555700001400570700002000584856006000604 2014 eng d00aJ.G.C. Lehmann's 'Botanical Observations' of 1818 on Coldenia, Colsmannia, Cynoglossum, and Omphalodes (commented translation)0 aJGC Lehmanns Botanical Observations of 1818 on iColdenia Colsman c2014/// ae10640 v23 a

An annotated English translation of a German early 19th century text including Latin diagnoses is presented with a high-quality scan of the original publication and direct links to the cited pages with taxon and literature citations (including TL-2 entries).

1 aHilger, HH1 aCole, TCH1 aSelvi, Federico uhttp://biodiversitydatajournal.com/articles.php?id=106401269nas a2200133 4500008004100000245009000041210006900131490000700200520083100207100001601038700001401054700002001068856004701088 2012 eng d00aNonea dumanii sp. nov. (Boraginaceae) from the Taurus mountains (south Turkey)0 aiNonea dumaniii sp nov Boraginaceae from the Taurus mountains so0 v303 a

The new species Nonea dumanii, endemic to the mountains of the western Taurus in south Turkey (C4 Antalya), is described
an illustrated based on original collections by the authors. Karyological observations and analysis of ITS1 DNA sequences
showed that the species is probably hexaploid with 2n = 6x = 60 and has phylogenetic affinity to the diploid N. monticola
from the Paphlagonian mountains as well as to the tetraploid N. anchusoides from northwest Iran and southeast Turkey.
From these allopatric species it is morphologically distinct in characters of the indumentum, flower and fruit. Polyploidy
is supported as a major driving force for speciation in Nonea, especially in the group of Anatolian mountain species with
primary base number x =10.
 

1 aBilgili, B.1 aCoppi, A.1 aSelvi, Federico uhttps://boraginales.myspecies.info/node/3703131nas a2200253 4500008004100000245014200041210006900183490000700252520235900259653001202618653001102630653001902641653003202660653001202692653001102704653001302715653001902728653001402747100001602761700002002777700001902797700001402816856004702830 2009 eng d00aOrigin of Mediterranean insular endemics in the Boraginales: integrative evidence from molecular dating and ancestral area reconstruction0 aOrigin of Mediterranean insular endemics in the Boraginales inte0 v363 a

Aim The presence of numerous reliable fossils and the occurrence of many
endemic island species make the Boraginales particularly suitable for integrative
biogeographical studies. In this paper we aim to elucidate the time frame
and events associated with the origin of selected borages endemic to the
Mediterranean climate zone. More specifically, we describe and examine the
alternative palaeo- and neoendemic hypotheses for their origin.
Location Corsica and Sardinia (continental fragment islands) and the Canary
Islands (an oceanic island archipelago).
Methods Eighty-nine accessions, representing 30 genera from five families
ascribed to the Boraginales, were examined for six chloroplast DNA regions. We
used an integrative approach including phylogenetic analyses (MrBayes),
Bayesian molecular dating (T3 package) with four fossil constraints on nodes,
and biogeographical reconstructions (diva) to elucidate the temporal and spatial
origins of the Corso-Sardinian and Canary Island endemics.
Results Species of Echium endemic to the Canary Islands diverged from their
continental sister clade during the Miocene (15.3 ± 5.4 Ma), probably after the
rise of the oldest islands (c. 20 Ma). Corso-Sardinian endemics of Borago
diverged from their primarily North African sister clade during the late
Miocene-Pliocene (c. 6.9 ± 3.6 Ma), well after the initial fragmentation of the
islands (c. 30 Ma). Similarly, Corso-Sardinian endemics of Anchusa diverged
from the South African Anchusa capensis during the Pliocene–Pleistocene (c. 2.7 ± 2.1 Ma).

Main conclusions The present study reveals an Anatolian origin for Anchusa,
Borago and Echium and underlines the importance of the Eastern Mediterranean
region as a possible reservoir for plant evolution in the Mediterranean Basin. For
Anchusa and Borago, the divergence from their respective sister clades on the two
types of islands post-dated the formation of the islands, thus supporting the neo-
endemic hypothesis, whereas the dating results for the origin of Echium endemics
were less conclusive.
 



 

10aAnchusa10aBorago10aCanary Islands10acontinental fragment island10aCorsica10aEchium10aendemism10aoceanic island10aSardinia.1 aMansion, G.1 aSelvi, Federico1 aGuggisberg, A.1 aConti, E. uhttps://boraginales.myspecies.info/node/3602636nas a2200193 4500008004100000245023100041210006900272490000800341520190000349653001702249653002002266653001302286653002402299653001602323653002602339100001502365700002002380856004202400 2009 eng d00aPhylogenetic relationships of the monotypic genera Halacsya and Paramoltkia and the origins of serpentine adaptation in circum- mediterranean Lithospermeae (Boraginaceae): insights from ITS and matK DNA sequences0 aPhylogenetic relationships of the monotypic genera iHalacsyai an0 v58 3 a

Halacsya and Paramoltkia are monotypic and partially sympatric genera in the Balkans, with no clear relation-
ships among extant Lithospermeae due to striking morphological autapomorphies and scarcity of phylogenetic
analyses in this group. The two species H. sendtneri and P. doerfleri show a strict selectivity for serpentine soils,
posing the question whether this edaphic specialization reflects a common ancestry or a parallel process of adap-
tive evolution in unrelated lineages. DNA sequences from the nuclear ITS and chloroplast matK regions were
generated from multiple accessions of Halacsya and Paramoltkia, and from representatives of 16 other genera
of Mediterranean Lithospermeae. SEM analyses of pollen morphology were also conducted to test relationships
indicated by molecular phylogenies. Parsimony analyses retrieved a clade of morphologically well differentiated
monotypic Lithospermeae including Halacsya and Paramoltkia. ITS provided a better resolution of relationships
and showed the two genera to be sistergroups close to Mairetis and Moltkiopsis, and no affinity to Moltkia as sup-
posed by past authors. Pollen characters corroborated the phylogenetic link between the two Balkan genera. Five
further monophyletic clades were recognised: Onosma-Echium, Moltkia, Lithospermum s.l., Arnebia-Macrotomia,
and Alkanna-Podonosma. Mapping the edaphic preferences of Lithospermeae onto molecular cladograms showed
that serpentinophytism as an obligate condition originated separately in the clade of monotypic genera and in
that of Onosma-Echium. In Halacsya and Paramoltkia it represents an early ecological synapomorphy which
probably originated in situ from non-serpentine ancestors related to Moltkiopsis and Mairetis.

 

10aBoraginaceae10aedaphic habitat10aHalacsya10amolecular phylogeny10aParamoltkia10aserpentine adaptation1 aCecchi, L.1 aSelvi, Federico u http://www.jstor.org/stable/2775693903067nas a2200145 4500008004100000245015700041210006900198490000700267520239900274653011602673100002002789700001402809700001502823856008302838 2011 eng d00aHigh epizoochorous specialization and low DNA sequence divergence in Mediterranean Cynoglossum (Boraginaceae): Evidence from fruit traits and ITS region0 aHigh epizoochorous specialization and low DNA sequence divergenc0 v603 a

Fruit morpho-anatomy and DNA sequence diversity in Euro-Mediterranean taxa of Cynoglossum and the closely
related genera Solenanthus and Pardoglossum were analysed to assess the structural traits that promote dispersal through
transport via the fur of mammals, and to evaluate the phylogenetic value of carpological variation in the group. Electron and
light microscopy showed striking epizoochorous adaptations in characters of the pluricellular projections of the epicarp (glo-
chids), such as multiple apical hooks, conical shape, and finely tuberculate surface, as well as the heavy mineralization of the
cell walls with especially silicon and calcium revealed by X-ray microanalysis. The attachment potential of nutlets to sheep
fleece, estimated through a General Linear Model, was species-specific and relatively high in the taxa with small and light
diaspores (> 60%). ITS sequences from 29 specific and infraspecific accessions were poorly variable, with pairwise genetic
distances ranging from 0.002 to 0.097 (mean 0.044). A comparative analysis of ITS sequence diversity in relation to the differ-
ent dispersal strategies in the four main Boraginaceae tribes revealed substantially higher levels of variation and interspecific
genetic distances in the non-epizoochorous groups, including the Cynoglosseae genera Myosotis and Omphalodes. Bayesian and
maximum parsimony tree construction suggested paraphyly of Cynoglossum due to the nested position of Solenanthus apen-
ninus and Pardoglossum. Species-level relationships remained largely unresolved, preventing an estimation of the phylogenetic
significance of fruit characters. Rapid spread across the Mediterranean region via epizoochory by wild mammals is possibly
the primary cause for the lack of genetic divergence among species. During the Holocene, nomadic and transhumant pasture
by domesticated herbivores has likely contributed to further spreading and mixing of previously isolated taxa, possibly causing
events of homoploid hybridization and introgression. More variable molecular markers should be tested to better understand
the impact of these processes in the evolutionary history of Cynoglossum and to solve relationships within Cynoglosseae.

 

10aBoraginaceae; Cynoglossum; epizoochory; fruit morphology; ITS sequences; phylogeny; seed dispersal; systematics1 aSelvi, Federico1 aCoppi, A.1 aCecchi, L. uhttp://www.ingentaconnect.com/content/iapt/tax/2011/00000060/00000004/art0000301875nas a2200193 4500008004100000245007700041210006900118490000800187520130900195653001701504653001601521653001801537653001401555653001601569653001301585100002001598700001601618856004701634 2012 eng d00aA synopsis of the genus Cynoglossum (Boraginaceae-Cynoglosseae) in Italy0 asynopsis of the genus Cynoglossum BoraginaceaeCynoglosseae in It0 v1463 a

Based on field and herbarium investigations, a systematic synthesis of the critical genus Cynoglossum (Boraginaceae) in Italy is
provided. Types, diagnostic characters and original iconographies are given for each native species, together with a revised
analytical key. Regional distributions are outlined and a list of selected vouchers is included. Nine species are native to the
Italian territory: Cynoglossum barbaricinum, Cynoglossum cheirifolium, Cynoglossum clandestinum, C. columnae, C. creticum, C.
magellense, C. montanum, C. nebrodense and C. officinale. A new subspecies, named C. nebrodense ssp. lucanum, is described
from some calcareous massifs in S Basilicata and N Calabria. This taxon is morphologically and geographically distinct from
the typical C. nebrodense s.str. In addition, C. dioscoridis and C. germanicum, often reported from Italy, cannot be confirmed
and are apparently to be excluded from the Italian flora. Old herbarium records of C. amabile Stapf & Drummond and C.
coelestinum Lindb. [: Paracaryopsis coelestina (Lindl.) R.R. Mill] from northeast Italy are reported for the first time, although
the presence of these two exotic species in Italy was probably occasional.
 

10aBoraginaceae10aCynoglossum10aItalian flora10akaryology10asystematics10aTaxonomy1 aSelvi, Federico1 aSutorý, K. uhttps://boraginales.myspecies.info/node/3302329nas a2200205 4500008004100000245007800041210006900119490000700188520173000195653001701925653001301942653002501955653002401980653001602004653001302020100002002033700001502053700001402068856004102082 2009 eng d00aPhylogeny, karyotype evolution and taxonomy of Cerinthe L. (Boraginaceae)0 aPhylogeny karyotype evolution and taxonomy of Cerinthe L Boragin0 v583 a

A phylogenetic and karyological analysis of the small and poorly known genus Cerinthe L. (Boraginaceae-
Lithospermeae) was performed using ITS sequences and standard chromosomal techniques. All taxa are
diploid with 2n = 16 or 2n = 18 and show a variable degree of infraspecific variation, in particular in the
polymorphic C. major and C. minor. Change in base number is associated with an early split between the
two well-supported clades of C. major, corresponding to Cerinthe sect. Cerinthe, and that of all other taxa
belonging to C. sect. Ceranthe, with the base x = 8 found only in the strictly annual C. major group, and
x = 9 in the other five species of the genus: C. minor, C. glabra, C. tenuiflora, C. retorta and C. palaestina.
The latter section is subdivided into the E Mediterranean, annual lineage of C. palaestina–C. retorta and
the mainly continental, perennial group of C. minor–C. glabra, the sister of which is the Corsican endemic
C. tenuiflora. The hypothesis that x = 9 represents the primary haploid number and x = 8 is derived through
descending aneuploidy, is discussed. A taxonomic revision of the genus is provided and the following formal
taxonomic changes are proposed: C. major L. subsp. oranensis (Batt.) Selvi & Cecchi, stat. nov.; C. major
L. subsp. purpurascens (Boiss.) Selvi & Cecchi, stat. nov.; C. minor L. subsp. cleiostoma (Boiss. & Sprun.)
Selvi & Cecchi, stat. nov. Cerinthe tenuiflora, C. retorta and the poorly known C. palaestina are well-defined
species with little internal variation.

 

10aBoraginaceae10aCerinthe10achromosome evolution10amolecular phylogeny10asystematics10aTaxonomy1 aSelvi, Federico1 aCecchi, L.1 aCoppi, A. uhttp://www.jstor.org/stable/2775701900580nas a2200169 4500008004100000245012600041210006900167260001200236300001400248490000700262520000900269100001600278700002200294700002000316700001500336856005900351 2010 eng d00aFossil and extant Western Hemisphere Boragineae, and the polyphyly of "Trigonotideae" Riedl (Boraginaceae: Boraginoideae)0 aFossil and extant Western Hemisphere Boragineae and the polyphyl c2010/// a409 - 4190 v353 anull1 aWeigend, M.1 aGottschling, Marc1 aSelvi, Federico1 aHilger, HH uhttp://www.bioone.org/doi/abs/10.1600/036364410X51887400754nas a2200169 4500008004100000245016200041210006900203260001200272300001400284490000700298653010600305100001600411700002200427700002000449700001500469856010000484 2009 eng d00aMarbleseeds are gromwells - Systematics and evolution of Lithospermum and allies (Boraginaceae tribe Lithospermeae) based on molecular and morphological data0 aMarbleseeds are gromwells Systematics and evolution of Lithosper c2009/// a755 - 7680 v5210aAmerica Fruit Historical biogeography Macromeria Nomosa Onosmodium Phylogeny Pollen morphology Timing1 aWeigend, M.1 aGottschling, Marc1 aSelvi, Federico1 aHilger, HH uhttp://www.sciencedirect.com/science/article/B6WNH-4WBK7BK-3/2/5ef424fa3684d5c985634011154460d503458nas a2200229 4500008004100000020001400041245015600055210006900211260001400280300001400294490000700308520269000315653001703005653002203022653001903044100001603063700002203079700002003101700002103121700001503142856007103157 2013 eng d a1055-790300aMultiple origins for Hound’s tongues (Cynoglossum L.) and Navel seeds (Omphalodes Mill.) – The phylogeny of the borage family (Boraginaceae s.str.)0 aMultiple origins for Hound s tongues Cynoglossum L and Navel see c2013/09// a604 - 6180 v683 aAbstractRecent studies all indicated that both the affinities and subdivision of Boraginaceae s.str. are unsatisfactorily resolved. Major open issues are the placement and affinities of Boraginaceae s.str. in Boraginales and the major clades of the family, with especially the large tribes Cynoglosseae and Eritrichieae repeatedly retrieved as non-monophyletic groups, and the doubtful monophyly of several larger genera, especially Cynoglossum and Omphalodes. The present study addresses and solves these questions using two plastid markers (trnL–trnF, rps16) on the basis of a sampling including 16 outgroup taxa and 172 ingroup species from 65 genera. The phylogeny shows high statistical support for most nodes on the backbone and on the individual clades. Boraginaceae s.str. are sister to African Wellstediaceae, Wellstediaceae–Boraginaceae s.str. is sister to African Codonaceae. Echiochileae are retrieved as sister to the remainder of Boraginaceae s.str., which, in turn, fall into two major clades, the Boragineae–Lithospermeae (in a well-supported sister relationship) and the Cynoglosseae s.l. (including Eritrichieae). Cynoglosseae s.l. is highly resolved, with Trichodesmeae (incl. Microcaryum, Lasiocaryum) as sister to the remainder of the group. Eritrichieae s.str. (Eritrichium, Hackelia, Lappula) are resolved on a poorly supported polytomy together with the Omphalodes-clade (incl. Myosotidium, Cynoglossum p.p.), and the Mertensia-clade (incl. O. scorpioides, Asperugo). The Myosotideae (Myosotis, Trigonotis, Pseudomertensia) are retrieved in a well-supported sister-relationship to the core-Cynoglosseae, the latter comprising all other genera sampled. Cynoglossum is retrieved as highly para- and polyphyletic, with a large range of generic segregates embedded in Cynoglossum, but other species of Cynoglossum are sister to Microula or to the American “Eritrichieae” (Cryptantha and allied genera). Representatives of the genus Cynoglossum in its current definition are segregated onto six independent lineages, members of Omphalodes onto three independent lineages. At least 11 of the genera here sampled are deeply nested in other genera. The data show that individual details of nutlet morphology (e.g., winged margins, glochidia) are highly homoplasious. Conversely, a complex of nutlet characters (e.g., characters of the gynobase and cicatrix together with nutlet orientation and sculpturing) tends to circumscribe natural units. Geographical distribution of major clades suggests that the family originated in Africa and western Asia and radiated to eastern Eurasia, with several independent dispersal events into Australia and the New World. 10aBoraginaceae10aNutlet morphology10aPhytogeography1 aWeigend, M.1 aLuebert, Federico1 aSelvi, Federico1 aBrokamp, Grischa1 aHilger, HH uhttp://www.sciencedirect.com/science/article/pii/S1055790313001590